Journal: bioRxiv

Article Title: Plasma-Enabled Multiscale Coupling of Architecture and Biointerfaces Drives Osteogenesis in 3D-Printed Gyroid Scaffolds

doi: 10.64898/2026.04.16.718992

Figure Lengend Snippet: Fabrication and surface characterization of silver nanoparticle (AgNP)-functionalized PLA scaffolds. A) Schematic illustration of the scaffold fabrication and surface modification workflow: hexagonal honeycomb G-code was used to 3D-print PLA scaffolds, which were subsequently dip-coated in AgNO₃ solution, with or without prior incubation in polydopamine hydrochloride (PDA), followed by Plasma Electroless Reduction (PER) under H₂ gas to yield PLA+AgNP and PLA+PDA+AgNP constructs, respectively. B) Representative scanning electron microscopy (SEM) images of PLA+AgNP (top row) and PLA+PDA+AgNP (bottom row) scaffolds fabricated across a range of AgNO₃ concentrations (0–25 mM), with corresponding optical images of the scaffold surface shown as insets. Scale bars = 5 µm. C) SEM micrographs of L929 fibroblasts (top row) and human mesenchymal stem cells (hMSCs, bottom row) adhered to unmodified PLA HC, PLA HC+AgNP (0.7 mM AgNO₃), and PLA HC+PDA+AgNP (0.7 mM AgNO₃) scaffolds. Black arrows indicate representative cell–scaffold interactions. Scale bars = 15 µm.

Article Snippet: The cells were incubated in mesenchymal stem cell complete media (Basal media: ATCC, USA: Cat no: PCS-500-030 and growth kit: ATCC, USA: Cat no: PCS-500-041) for five days.

Techniques: Modification, Incubation, Clinical Proteomics, Construct, Electron Microscopy